Inter-simple Sequence Repeat (ISSR) Analysis of Somaclonal Variation in Artemisia amygdalina Decne. plantlets Regenerated from nodal explants
نویسندگان
چکیده
In this study Artemisia amygdalina a critically endangered medicinal plant endemic to Kashmir Himalayas was grown under in vitro conditions. ISSR markers were used to study the somaclonal variation at the genetic level. Two ISSR-primers (CAC)3 GC and (ACTG)5 were used, two additional DNA bands (0.15kb and 0.2kb) were observed respectively in tissue cultured Artemisia amygdalina. Direct regeneration was seen from nodal explants on MS + BAP (10μM) + (NAA (10μM). The results depict that direct regenerants were actually the somaclonal variants but not true to type as was expected.
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